Standard Operating Procedure for Determination of Lignin Structure with NMR Spectroscopy Within the Nanostruc-turome Methods Pipeline

Avtorji

Jan Hočevar
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia
Grigorij Prinčič Griša
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia
Jernej Iskra
Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia

Kratka vsebina

Standard operating procedure (SOP) for the characterization of Organosolv lignin from wood using nuclear magnetic resonance (NMR) spectroscopy is presented. The aim of this procedure within the Nanostructurome methods pipeline is to determine the structural composition of lignin, including the relative proportions of syringyl (S), guaiacyl (G), and p-hydroxyphenyl (H) units, as well as the distribution of interunit linkages such as β-O-4, β-β, and β-5 bonds. The extracted lignin was analyzed using 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy. This method enables precise structural elucidation of signals from aromatic and aliphatic regions. The mathematical model quantifies the abundance of aromatic units and in-terunit linkages, allowing for comparison of lignin fractions. Proper laboratory prac-tices, including chemical handling, equipment maintenance, and data management, are outlined to ensure the reliability and reproducibility of results. Health and safety measures are emphasized, particularly regarding the disposal of byproducts such as black liquor and solvent waste. The SOP serves as a valuable guideline for lignin structural analysis, facilitating its application in biorefinery processes, material devel-opment, and bioengineering.

Prenosi

Izdano

10 June 2025

Kako citirati

Hočevar, J., Prinčič Griša, G., & Iskra, J. (2025). Standard Operating Procedure for Determination of Lignin Structure with NMR Spectroscopy Within the Nanostruc-turome Methods Pipeline. In V. Kralj-Iglič, Y. Istileulova, & A. Romolo (Eds.), Socratic Lectures 12 th International Symposium, Ljubljana, January 11, 2025: PART III (pp. 80–88). Založba Univerze v Ljubljani. https://doi.org/10.55295/PSL.12.2025.III10