Standard Operating Procedure for Determination of Lignin Structure with NMR Spectroscopy Within the Nanostruc-turome Methods Pipeline
Kratka vsebina
Standard operating procedure (SOP) for the characterization of Organosolv lignin from wood using nuclear magnetic resonance (NMR) spectroscopy is presented. The aim of this procedure within the Nanostructurome methods pipeline is to determine the structural composition of lignin, including the relative proportions of syringyl (S), guaiacyl (G), and p-hydroxyphenyl (H) units, as well as the distribution of interunit linkages such as β-O-4, β-β, and β-5 bonds. The extracted lignin was analyzed using 2D heteronuclear single quantum coherence (HSQC) NMR spectroscopy. This method enables precise structural elucidation of signals from aromatic and aliphatic regions. The mathematical model quantifies the abundance of aromatic units and in-terunit linkages, allowing for comparison of lignin fractions. Proper laboratory prac-tices, including chemical handling, equipment maintenance, and data management, are outlined to ensure the reliability and reproducibility of results. Health and safety measures are emphasized, particularly regarding the disposal of byproducts such as black liquor and solvent waste. The SOP serves as a valuable guideline for lignin structural analysis, facilitating its application in biorefinery processes, material devel-opment, and bioengineering.
Prenosi
Pages
Izdano
Kategorije
Licenca

To delo je licencirano pod Creative Commons Priznanje avtorstva-Deljenje pod enakimi pogoji 4.0 mednarodno licenco.