Standard Operating Procedure for Quantifying Growth and In-flammatory Factors in Cell Culture Supernatants, and Plasma via ELISA within the Nanostructurome Methods Pipeline
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Here we present the Standard operating procedure (SOP) used used to quantify growth factors and inflammatory markers in extracellular vesicles (EVs) - containing cell cul-ture supernatants, plasma samples and using enzyme linked Immunosorbent assay (ELISA). The targets investigated in plassma and supernatant include interleukin 6 (IL-6), tumor necrosis factor α (TNFα), platelet-derived growth factor-BB (PDGF-BB), transforming growth factor β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF). These soluble mediators are crucial for cell proliferation, differentiation, and immune response regulation. They may also play a pivotal role in determining the efficacy of EV-based regenerative therapies. This SOPs was implemented within the Nanostructurome methods pipeline.
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