Standard Operating Procedure for Quantifying Growth and In-flammatory Factors in Cell Culture Supernatants, and Plasma via ELISA within the Nanostructurome Methods Pipeline

Authors

Sara Michelini
University of Ljubljana, Biotechnical faculty, Department of Biology, Ljubljana, Slovenia
Damjana Drobne
University of Ljubljana, Biotechnical faculty, Department of Biology, Ljubljana, Slovenia

Synopsis

Here we present the Standard operating procedure (SOP) used used to quantify growth factors and inflammatory markers in extracellular vesicles (EVs) - containing cell cul-ture supernatants, plasma samples and using enzyme linked Immunosorbent assay (ELISA). The targets investigated in plassma and supernatant include interleukin 6 (IL-6), tumor necrosis factor α (TNFα), platelet-derived growth factor-BB (PDGF-BB), transforming growth factor β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF). These soluble mediators are crucial for cell proliferation, differentiation, and immune response regulation. They may also play a pivotal role in determining the efficacy of EV-based regenerative therapies. This SOPs was implemented within the Nanostructurome methods pipeline.

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Published

June 10, 2025

How to Cite

Michelini, S., & Drobne, D. (2025). Standard Operating Procedure for Quantifying Growth and In-flammatory Factors in Cell Culture Supernatants, and Plasma via ELISA within the Nanostructurome Methods Pipeline. In V. Kralj-Iglič, Y. Istileulova, & A. Romolo (Eds.), Socratic Lectures 12 th International Symposium, Ljubljana, January 11, 2025: PART III (pp. 25–31). University of Ljubljana Press. https://doi.org/10.55295/PSL.12.2025.III4