Standard Operating Procedure for Quantifying Growth and In-flammatory Factors in Cell Culture Supernatants, and Plasma via ELISA within the Nanostructurome Methods Pipeline
Synopsis
Here we present the Standard operating procedure (SOP) used used to quantify growth factors and inflammatory markers in extracellular vesicles (EVs) - containing cell cul-ture supernatants, plasma samples and using enzyme linked Immunosorbent assay (ELISA). The targets investigated in plassma and supernatant include interleukin 6 (IL-6), tumor necrosis factor α (TNFα), platelet-derived growth factor-BB (PDGF-BB), transforming growth factor β (TGF-β), epidermal growth factor (EGF), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF). These soluble mediators are crucial for cell proliferation, differentiation, and immune response regulation. They may also play a pivotal role in determining the efficacy of EV-based regenerative therapies. This SOPs was implemented within the Nanostructurome methods pipeline.
Downloads
Pages
Published
License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.