Preparation of Size-Controlled Unilamellar Lipid Vesicle Suspension from Lipid Powder: A Standard Operating Pro-cedure within the Nanostructurome Methods Pipeline
Synopsis
Liposomes, owing to their high biocompatibility and ease of production and ma-nipulation, are now largely employed as nanocarriers in drug delivery and as sim-plified cell-membrane models in biophysics for the study of their physical, struc-tural, and nanomechanical properties. To ensure reliable results and sample stabil-ity, liposomes are most of the time unilamellar and of a specific size range. In this proceeding paper, some widely used process steps will be combined to form a complete methodology for forming unilamellar vesicles of defined diameter.
Downloads
Pages
11–17
Published
June 10, 2025
Copyright (c) 2025 Veronika Kralj-Iglič (Urednik zbornika); Eirini Andreasidou, David Heath, Marija Tanović, Isidora Santrač, Milena Dimitrijević, Valentina Ćurić, Snežana Kovačević, Marina Stanić, Jelena Danilović Luković, Concetta D’Antonio, Francesca Mantile, Giovanna L Liguori, Jan Hočevar, Grigorij Prinčič Griša, Jernej Iskra, Romana Cerc Korošec, Urška Lavrenčič Štangar, Ester Heath, Rok Mihelič, Eva Cepec; Yelena Istileulova, Anna Romolo (Urednik zbornika); Tjaša Griessler-Bulc, Urška Šunta, Darja Istenič, Laure Bar, Marta Lavrič, Miona Kovachevikj, Jernej Jakše, Taja Jeseničnik, Sara Michelini, Damjana Drobne, Tanja Zrnec Drobnjak, Lara Resman, Vid Žitko, Ana Schwarzmann, Lara Oprešnik
License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
How to Cite
Bar, L., & Lavrič, M. (2025). Preparation of Size-Controlled Unilamellar Lipid Vesicle Suspension from Lipid Powder: A Standard Operating Pro-cedure within the Nanostructurome Methods Pipeline. In V. Kralj-Iglič, Y. Istileulova, & A. Romolo (Eds.), Socratic Lectures 12 th International Symposium, Ljubljana, January 11, 2025: PART III (pp. 11–17). University of Ljubljana Press. https://doi.org/10.55295/PSL.12.2025.III2
