Standard Operating Procedure for Interferometric Light Mi-croscopy of Extracellular Particles within the Nanostructurome Pipeline
Synopsis
A standard operating procedure (SOP) for assessment of number density and hy-drodynamic diameter of particles sized between cca 80 nm and 500 nm is presented. The aim of this SOP is to provide characterization of extracellular particles (EPs) which enables analysis of large cohorts of minimally processed samples. EPs are sub-micron sized particles shed by all types of cells. They are free to travel to reach dis-tant cells and can interact with them. Therefore they present an universal mechanism of cell-to-cell communication which is a subject of studies of different mechanisms underlying physiological and pathophysiological processes.
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Pages
49–57
Published
June 10, 2025
Copyright (c) 2025 Veronika Kralj-Iglič (Urednik zbornika); Eirini Andreasidou, David Heath, Marija Tanović, Isidora Santrač, Milena Dimitrijević, Valentina Ćurić, Snežana Kovačević, Marina Stanić, Jelena Danilović Luković, Concetta D’Antonio, Francesca Mantile, Giovanna L Liguori, Jan Hočevar, Grigorij Prinčič Griša, Jernej Iskra, Romana Cerc Korošec, Urška Lavrenčič Štangar, Ester Heath, Rok Mihelič, Eva Cepec; Yelena Istileulova, Anna Romolo (Urednik zbornika); Tjaša Griessler-Bulc, Urška Šunta, Darja Istenič, Laure Bar, Marta Lavrič, Miona Kovachevikj, Jernej Jakše, Taja Jeseničnik, Sara Michelini, Damjana Drobne, Tanja Zrnec Drobnjak, Lara Resman, Vid Žitko, Ana Schwarzmann, Lara Oprešnik
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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
How to Cite
Kralj-Iglič, V., Istileulova, Y., & Romolo, A. (Eds.). (2025). Standard Operating Procedure for Interferometric Light Mi-croscopy of Extracellular Particles within the Nanostructurome Pipeline. In Socratic Lectures 12 th International Symposium, Ljubljana, January 11, 2025: PART III (pp. 49–57). University of Ljubljana Press. https://doi.org/10.55295/PSL.12.2025.III7
