Biological Effects of Plant Cannabinoids on Saccharomyces cerevisiae Assessed Using the Kirby-Bauer Disk Diffusion Method

Authors

Maja Ponikvar-Svet, Jožef Stefan Institute, Department of Inorganic Chemistry and Technology, Ljubljana; Sergio Sánchez Bruni, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA) and Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET-UNCPBA-CICPBA, Tandil, Buenos Aires, Argentina; Maja Kitek, Jožef Stefan Institute, Department of Inorganic Chemistry and Technology, Ljubljana; Maja Satošek, Jožef Stefan Institute, Department of Inorganic Chemistry and Technology, Ljubljana; Laureano Schofs, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA) and Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET-UNCPBA-CICPBA, Tandil, Buenos Aires, Argentina; Luka Irenej Pečan, GaiaCell, Advanced Cell and Gene Therapy Ltd., Trzin, Slovenia; Gašper Tavčar, Jožef Stefan Institute, Department of Inorganic Chemistry and Technology, Ljubljana; Marko Jeran, Jožef Stefan Institute, Department of Inorganic Chemistry and Technology, Ljubljana

Synopsis

Cannabis sativa L. subsp. sativa, commonly known as hemp, has historically been used for its nutritional, industrial, and medicinal properties. The plant produces cannabinoids such as cannabidiol (CBD), which have shown therapeutic effects, including anti-inflammatory, anxiolytic, and analgesic properties. This study examines the antimicrobial potential of CBD and a complete hemp isolate against Saccharomyces cerevisiae, a model yeast organism. Using the solid-state diffusion method, different concentrations of CBD and hemp isolate were applied to S. cerevisiae cultures. Results showed that both CBD and hemp isolate displayed varying levels of antimicrobial activity, with the most significant effects observed at higher concentrations (500 μg/mL) after 24 hours of exposure. The study suggests that cannabinoids may provide promising natural antifungal agents for further research and application in biotechnology and pharmaceuticals.

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Volume

Socratic Lectures 14th International Symposium, Ljubljana, April 17, 2026 Part IV– Interdisciplinary Science: Part IV– Interdisciplinary Science

Pages

20-27

Published

August 5, 2026

Categories

Copyright (c) 2026 Veronika Rihter, Andrej Race, Venkata Subba Rao Jampani, Marko Novinec, Ivan Jerman, Rezki Derni; Nadjib M. Rahmoun; Abdellah Mertoumi, Polona Hrovat, Sabina Knez, Jan Dolinar, Ana Obaha, Valentina Ćurić, Nina Šubelj, Raja Gošnak Dahmane, Jelena Danilović Luković; Anna Romolo (urednik zbornika); Matej Hočevar, Darja Feizpour, Barbara Šetina Batič, Tina Sever, Isidora Santrač, Marija Tanović, Maša Kramer, Ela Nusdofer, Tine Oblak, Maja Ponikvar-Svet, Jaša Petek, Tai Klampfer, Said Bešlagić, Ajda Ravnihar, Katarina Jugovic, Karin Kunstelj, Patricija Lap, Eva Merklin, Anej Kramer, Tara Sember, Raja Gošnak Dahmane, Eva Juvančič, Natalija Kodrnja, Tjaša Šuc, Kaja Tomasino, Miona Kovachevikj, Taja Jeseničnik, Jernej Jakše, Luka Irenej Pečan, Ines Malek; Veronika Kralj-Iglič (urednik zbornika); Monika Jenko, Maja Ovsenik, Mojca Debeljak, Matevž Arko, Boštjan Korenjak, Aleš Iglič, Petra Bezeljak Cerv, Anita Prelovšek, Gregory T Papanikos, Daniela Brogna, Jasna Majstorović, Sergio Sánchez Bruni; Yelena Istileulova (urednik zbornika); Neda Bošković, Almedina Kavčič, Maja Kitek, Maja Satošek, Laureano Schofs, Luka Irenej Pečan, Gašper Tavčar, Hatice Kırmacı, Arzu Çalik Seydim, Andreja Abina, Aleksander Zidanšek, Marko Jeran, Eva Bevc, Lia Cuogo, Tiana Janković, Anja Boc, Maruša Blaško, Jasna Kastivnik, Vita Zbačnik, Esra Tuğçe Bal, Duygu Güney, Boris Cizelj, Wayan Kun Adnyana, Nicoletta Huștiuc, Elena Bliznyuk, Irina Pozdorovkina, Lidiia Rezvukhina, Anna Zuykova, Žiga Babnik, Sara Kavazović

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How to Cite

Ponikvar-Svet, M., Sánchez Bruni, S., Kitek, M., Satošek, M., Schofs, L., Pečan, L. I., Tavčar, G., & Jeran, M. (2026). Biological Effects of Plant Cannabinoids on Saccharomyces cerevisiae Assessed Using the Kirby-Bauer Disk Diffusion Method. In A. Romolo, V. Kralj-Iglič, & Y. Istileulova (Eds.), & N. M. Rahmoun, Socratic Lectures 14th International Symposium, Ljubljana, April 17, 2026 Part IV– Interdisciplinary Science: Part IV– Interdisciplinary Science (pp. 20-27). University of Ljubljana Press. https://ebooks.uni-lj.si/ZalozbaUL/catalog/book/946/chapter/4877